mRNA Chemistry & Directed Immune Activation
Antigen Identification
Lipid Chemistry & Delivery Formulation
Process Engineeering

4 PLATFORMS

1. mRNA Chemistry & Directed Immune Activation

  • Optimised mRNA chemistry to shorten production time / throughput
  • Improved vectors to increase mRNA expression
  • Proprietary TriMix technology based on long-standing foundational academic research — check out our TriMix factsheet
  • Expanding portfolio of immune stimulants beyond TriMix for use in selected disease settings

2. Antigen Identification

  • Investigating validated TAAs in mRNA form
  • Discovering novel, patentable antigens through network of research collaborations
  • Multiple antigens leveraged across single modular platform allows for fast follower programs

3. Lipid Chemistry & Delivery Formulation

  • Extension of lipid libraries
  • Novel formulation for different routes of administration — check out our Formulation factsheet.
  • Explore alternative routes of administration, e.g. intranasal
  • Targeted delivery
  • Novel lipid chemistries
  • Additional benefits in improving thermo-stability of final product
  • In house GMP facility
  • Novel DS processes: reduce time and cost and increase yield
  • Novel DP processes: increase reproducibility
  • Engineering programme to develop automated closed system
  • Novel lyophilisation technology to reduce need for cold chain storage & transport
  • In house GMP facility — check out our Manufacturing Capacity factsheet.
  • Novel DS processes: reduce time and cost and increase yield
  • Novel DP processes: increase reproducibility
  • Engineering programme to develop automated closed system
  • Novel lyophilisation technology to reduce need for cold chain storage & transport

More on TriMix & TetraMix

TriMix

View our TriMix factsheet for my detailed information.

TriMix boosts immune response through the dendritic cell pathway in a sustainable way. eTheRNA’s TriMix contains three naked mRNA molecules:
1. caTLR4: Activates the immune system as it evokes dendritic cells to present antigens to the CD4/CD8 T-cells
2. CD40L: Induces the dendritic cells to initiate the antigen-specific action of the CD4 T-cells
3. CD70: Induces the dendritic cells to initiate the immune systems of the CD8 T-cells

TriMix is unique in the way it uses these three mRNA molecules to induce the proliferation of T-cells into either mature helper T-cells or cytotoxic T-cells – the ultimate ‘soldiers’ of the immune system that fight cancer cells and infectious agents. By combining TriMix with tumour-specific antigens or neoantigens the patient’s dendritic cells are stimulated to produce a more potent and larger population of antigen specific cytotoxic and helper T-cells compared to tumour antigens alone.

Initially, the TriMix technology was applied as an ex vivo product. The ex vivo product consists of dendritic cells extracted from the patient’s body, which are then, in the laboratory, modified with TriMix in combination with the tumour-specific antigen mRNA.

This cellular TriMix product is reinjected as an autologous product into the patient’s body.

In preclinical and phase I/IIa studies in advanced melanoma, the TriMix cellular ex vivo product – either as a standalone product or combined with a checkpoint inhibitor – was able to  induce dendritic cells to elicit a powerful immune response, which in turn resulted in a promising clinical response and a prolonged disease-free survival rate.

eTheRNA is now turning this ex vivo approach into products that can be directly injected intranodally in the form of naked mRNA or intravenously and intramuscularly, where the mRNA is packaged in lipid nanoparticles.

TetraMix

Second generation of mRNA-based adjuvant boosting the immune response through the dendritic cell pathway. Building on the experience with TriMix, a new mix of immunostimulatory mRNAs was designed with further increased T-cell activating properties against the co-administered antigens.

Galileilaan 19, 2845 Niel, Belgium

Our Address

Our Mailbox

+32 3 369 17 40

Our Phone

© 2020 Etherna | Studio Hanswijk