1 – Immunostimulation Technology
TriMix – Boosting immune response through the dendritic cell pathway in a sustainable way
eTheRNA’s TriMix contains three naked mRNA molecules:
TriMix is unique in the way it uses these three mRNA molecules to induce the proliferation of T-cells into either mature helper T-cells or cytotoxic T-cells – the ultimate ‘soldiers’ of the immune system that fight cancer cells and infectious agents. By combining TriMix with tumour-specific antigens or neoantigens the patient’s dendritic cells are stimulated to produce a more potent and larger population of antigen specific cytotoxic and helper T-cells compared to tumour antigens alone.
Initially, the TriMix technology was applied as an ex vivo product. The ex vivo product consists of dendritic cells extracted from the patient’s body, which are then, in the laboratory, modified with TriMix in combination with the tumour-specific antigen mRNA.
This cellular TriMix product is reinjected as an autologous product into the patient’s body.
eTheRNA is now turning this ex vivo approach into products that can be directly injected intranodally in the form of naked mRNA or intravenously, where the mRNA is packaged in lipid nanoparticles.
Lipid nanoparticles – intravenous and intramuscular administration of mRNA in a packaged form
Lipid nanoparticles are used as a formulation vehicle to deliver mRNA to the lymphoid organs where they can transfect dendritic cells after intravenous and intramuscular administration. This modality is currently in preclinical development with very strong protective immune responses observed in in-vivo models. The aim is also to induce a specific immune response against antigens presented as mRNA in the vaccine.
Additional immune stimulation is achieved with TriMix included into the product.
2 – Oncolysis and tumour microenvironment modulating technology
Naked or packaged mRNA is delivered intratumourally directly into the patient’s tumour site, a direct access to the effector site of the therapy. This modality offers opportunities to develop tumour-lytic, tumour-modifying and immune stimulating products. Tumour microenvironment modulating mRNA mix showed promising anti-tumour responses in the injected as well as in the non-injected tumours.
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